D600 automatic digital PCR system integrates three steps of digital PCR detection process: droplet generation, PCR amplification and fluorescence detection.
Fully automated experimental operation greatly reduces the testing time.
96 samples can be automatically detected within 3 hours, realizing a new digital PCR detection mode of "Sample in, result out".
- Digital PCR Technology :
Digital PCR(dPCR) technology is an absolute quantitative nucleic acid detection method, without the need for standard curve to accurately quantify nucleic acid, greatly ensuring the accuracy and precision of detection, is the third generation of quantitative nucleic acid detection technology after real-time fluorescence quantitative PCR technology. It has important application value in liquid biopsy, tumor concomitant diagnosis, noninvasive prenatal screening, pathogen load monitoring and so on.
- Absolute quantification :
No reference required
Independent of the standard curve.
- Accurate and sensitive :
High signal-to-noise ratio
Single copy can be tested.
- Reliable retest :
Good repeatability
Ensure the consistency of test results.
- High efficiency and stability :
Tolerance to inhibitors
Applicable to complex samples.
- Fast & uniformity :
Microdroplet array printing adopts the original vibration micro(OsciDrop®) core technology to rapidly and stably generate uniform nano-scale microdroplet.
- Stable amplification :
The nanodroplets are flattened into a single-layer array in the well plate for in-situ amplification and fluorescence reading detection. The operation is convenient and the nanodroplets are effectively used.
- Multiple fluorescence :
Nanodroplet array reading has high-performance LED excitation light source and orthogonal fluorescence imaging detection system, supports more fluorescence detection channels, and can develop super multiplex digital PCR detection system.
- Exact algorithm :
Using advanced plan-apochromatic fluorescence imaging correction technology and deep learning nanodroplet array recognition algorithm, the nanodroplet recognition accuracy is high and the signal-to-noise ratio is good.
* Original Vibration Micro(OsciDrop®) Core Technology :
- Fully Automatic :
The whole process of sampling, droplet generation, amplification and testing is realized.
- High throughput :
96 samples in three hours.
Suitable for different throughput requirements.
- Low cost :
Chip-free consumables.
Cost is reduced to qPCR level.
- Multiple channels :
Up to 6 channel fluorescence channels.
The product can be recycled separately.
- High precision :
In situ amplification and testing.
Improve accuracy and precision.
- Customization :
Number of nanodrops per sample.
It can be customized according to testing requirements.
- Configure PCR reaction system :
Reaction system:10～25μL.
- Nanodroplet generation :
Put the reaction system and consumables into the instrument for nanodroplet generation.
Each experiment can generate up to 96 samples; Each print and sample can generate up to 20,000 nanodroplets.
- PCR amplification reaction :
After the nanodroplets are generated, the instrument seals the amplification area of the well plate to complete the PCR reaction.
- Result analysis :
Fluorescence photo-detection is performed on each reaction well; the quantitative value of the sample is calculated according to the number of negative and positive nanodroplets, which is according to Poisson distribution.
- Quantitative detection of COVID-19 Coronavirus N gene and ORF1ab gene :
For the detection of the COVID-19 Coronavirus, Maccura digital PCR is highly sensitive, can quantitatively monitor changes in viral load, and improve the detection rate of weak positive samples. It plays an important role in viral load monitoring, re-examination after discharge and drug efficacy evaluation during the diagnosis and treatment of COVID-19 Coronavirus.
Nucleic acid standard reference is an important tool for national supervision of nucleic acid testing products. At present, digital PCR is the core method for the research and development of nucleic acid reference materials. As shown in the figure above, among the 9 companies participating in the COVID-19 Coronavirus national reference product calibration of the China National Institutes for Food and Drug Control, the COVID-19 Coronavirus reference calibration results reported based on Maccura digital PCR analysis system are the closest to the COVID-19 Coronavirus national reference product calibration results of the China National Institutes for Food and Drug Control.
- Absolute quantitative detection of single nucleotide mutation (SNV) :
EGFR gene mutation detection examples:EGFR gene is a receptor for cell proliferation and signal transduction of human epidermal growth factor. This gene is mutated in non-small cell lung cancer. Accurate detection of EGFR gene mutation is of great significance for drug selection in clinical treatment.
The above figure is a representative 2D scatter plot of human EGFR gene L858R, T790M and G719S detection.
* Detection kit :
- Menu :
HIV-1 Digital PCR Kit
2019-nCoV Digital PCR Kit
Influenza A Virus Digital PCR Kit
Influenza B Virus Digital PCR Kit
Rhinovirus Digital PCR Kit
Adenovirus Digital PCR Kit
Mycoplasma Pneumoniae Digital PCR Kit
Mycobacterium Tuberculosis Digital PCR Kit
Norovirus Digital PCR Kit
Enterovirus 71 Digital PCR Kit
Enterovirus Universal Digital PCR Kit
Coxsachie Virus A16 Digital PCR Kit
Monkeypox Virus Digital PCR kit
Streptococcus pneumoniae Digital PCR kit
Klebsiella pneumoniae Digital PCR kit
Pseudomonas aeruginosa Digital PCR kit
Acinetobacter baumannii Digital PCR kit
Staphylococcus aureus Digital PCR kit
Escherichia coli Digital PCR kit
Enterococcus faecium Digital PCR kit
Human EGFR T790M Digital PCR Kit
Human PIK3CA E542K, E545Q, H1047R Digital PCR Kit
- Customized Reagent :
Infection related- Respiratory pathogens 133 items
Infection related- Hand, foot, mouth and enterovirus pathogens 94 items
Infection related- Hepatitis virus 9 items
Infection related- Reproductive health 17 items
Infection related- Arthropod-borne disease 56 items
Infection related- Parasite 14 items
Tumor related- Methylation detection 12items
Tumor related- Mutation detection 52 items
Tumor related- Copy number variation 2 items
Tumor related- Fusion gene detection 4 items
Genetically related- Single gene disease 18 items
Animal related- Animal disease 12 items
Animal related- Zoonotic disease 10 items
Animal related- Pets 13 items
