The Wako HR series NEFA-HR(2) is an in vitro enzymatic colorimetric method assay for the quantitative determination of non-esterified fatty acids (NEFA) in serum.

Measurements of fatty acids are used in the diagnosis and treatment of various disorders of lipid metabolism. Labor intensive extraction/ titration methods have been commonly applied to the measurement of free fatty acids in serum. These approaches are time consuming, hazardous and not easily automated. The FUJIFILM Wako’s NEFA assay is an original test developed as an enzymatic method which is available in a series of individual reagents. The need for an extraction step has been eliminated. This enzymatic method is accurate, precise, simple and fast. The FUJIFILM Wako’s method relies upon the acylation of coenzyme A (CoA) and ultimately results in a purple colored product which can be measured colorimetrically at 550 nm.